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Cv14-zero background ptopo-ta cloning kit

WebZero Background pTOPO-TA Simple Cloning Kit. Regular price $200 00 $200.00. Anther size of the SKU#-1 171626. Regular price $500 00 $500.00. Zero Background pTOPO-TA Cloning Kit. Regular price $200 00 $200.00. pTOPO-D1 Directional Expression Kit. Regular price $450 00 $450.00. pTOPO-ENTR/D Directional Cloning Kit.

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WebCloning exploits this reaction to efficiently clone PCR products. Once the PCR product is cloned into the pcDNA ™3.4-TOPO® vector and the transformants are analyzed for correct orientation and reading frame, the expression plasmid may be transfected into your cell line of choice. Experiment Outline for Cloning WebZero Blunt TOPO PCR Cloning Kit options The Zero Blunt TOPO PCR Cloning Kit for direct insertion of blunt-ended PCR products into a plasmid vector can be purchased with a variety of competent cells that deliver different advantages depending upon your needs General cloning: TOP10 cells (Cat. No. K2800-20, K2800-40) High-efficiency cloning ... monash university careers website https://superiortshirt.com

CV14-Zero Background pTOPO-TA Cloning Kit （零背景TA克隆， …

WebThis kit is designed to clone cohesive or blunt-ended DNA fragments with a low background of non-recombinants. The technology described here is based on vectors containing the lethal E. coli gene, ccdB (Bernard, et al., 1994). The cloning vector, pZErO®-1, contains the ccdB gene fused to the C-terminus of LacZα. WebHere, we report a zero background TA cloning system that provides simple and high-efficiency direct cloning of PCR-amplified DNA fragments with almost no self-ligation. The improved T-vector system takes advantage of the restriction enzyme XcmI to generate a T-overhang after digestion and the negative selection marker gene ccdB to eliminate the ... http://www.aidlab.cn/up_product/big/2024-4-1-10395228493.pdf ibhotwe guest house

TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ …

Category:Zero Background pTOPO-TA Cloning Kit of Research Kits from …

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Cv14-zero background ptopo-ta cloning kit

Invitrogen Zero Blunt TOPO PCR Cloning Kit, without competent cells ...

http://aidlab.cn/products-show.asp?anclassid=91&nclassid=70&id=1839 WebThe TOPO“ TA Cloning¤ Kit is shipped on dry ice. Each kit contains a box with TOPO“ TA Cloning¤ reagents (Box 1) and a box with TOP10 or TOP10F« One Shot“ competent cells (Box 2). Store Box 1 at -20¡C and Box 2 at -80¡C. Types of TOPO“ TA Cloning¤ Kits Two different types of TOPO“ TA Cloning¤ Kits are available in two different ...

Cv14-zero background ptopo-ta cloning kit

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WebEach TOPO™ Cloning Kit for Sequencing contains pCR™4-TOPO™ or pCR™4Blunt-TOPO™ Vector, Salt Solution, dNTPs, Control Template … WebIntroduction. TA cloning is a simple method to clone any desirable fragment with an extra A (Adenine nucleotide) overhang into any linearized vector with T (Thymidine nucleotide) overhang. TOPO TA cloning is restriction digestion-free and ligation-free cloning. Tradition TA cloning method requires ligation step to seal the joints of the vector ...

http://fire.biol.wwu.edu/trent/trent/topota_man.pdf http://www.aidlab.com.cn/products-en.asp?anclassid=&nclassid=&page=2

WebFeatures of the Zero™ Blunt™ TOPO™ PCR Cloning Kits: Fast and easy— go from PCR to clones in just 3 steps and in as little as 5 … WebCV14-Zero Background pTOPO-TA Cloning Kit （零背景TA克隆，含多克隆酶切位点MCS） 点击次数：12858次 点击下载说明书 产品介绍： 本制品和传统的T4连接酶原理不 …

Webcell cannot grow normally, thereby achieving “zero” background. Containing a blunt end factor, 5 min TA/Blunt-Zero Cloning Kit is compatible with both TA clones and blunt clones. Components C601-01 (25 rxns) 5 × TA/Blunt-Zero Cloning Mixa 500 bp Control insert (20 ng/μl) M13 Primer Mix (10 μM)b 25 μl 5 μl 200 μl C601-02 (50 rxns) 2 × ...

WebApr 1, 2024 · 版本号:160320. ZeroBackgroundpTOPO-TACloningKit 零背景pTOPO-TA克隆试剂盒. 目录号：CV14 试剂盒组成、储存、稳定性： 试剂盒组成 20次(CV1401) 80 … ib hornerWebpCR2.1-TOPO Sequence and Map pCR2.1-TOPO Parental bacterial expression vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: I.M.A.G.E. Consortium Plasmids More Plasmid Sets monash university caulfield libraryWebCV14-Zero Background pTOPO-TA Cloning Kit [Manual Download] PC61-2 x SYBR Green qPCR Mix(High ROX) ... CV16-Zero Background pTOPO-Blunt Cloning Kit [Manual Download] 2 / 10 Pages Total 145Informations First / Previous / Next / … monash university chancelleryWebTOPO TA Cloningfi Kits are shipped on dry ice. Each kit contains a box with TOPO TA Cloningfi reagents (Box 1) and a box with One Shotfi Chemically Competent or ElectrocompŽ cells (Box 2). Store Box 1 at -20°C and Box 2 at -80°C. Types of TOPO TA Cloningfi Kits TOPO TA Cloningfi Kits are available with either pCRfi2.1-TOPOfi or ... ib houseWebHCV Genotype Panel (14 X 0.5 mL) Catalog# KZMC049. Log In to See Price. Product Information: Volume: 0.5mL. Units/Pack: 14. Storage Condition: -20°C or below. … ib hortWebThe Technology Behind TOPO Cloning Whether you’re PCR cloning with Taq DNA polymerase or a proofreading enzyme, there is a TOPO cloning kit available to take you quickly and efficiently into your downstream application. General subcloning Long PCR fragments In vitro transcription Sequencing Expression in E. coli Expression in … ibhousing finance shareWebTOPO® TA Cloning® Kit . Five-minute cloning of Taq polymerase-amplified PCR products . Catalog numbers (pCR ™ 2.1-TOPO ® vector) K4500-01, K4500-40, K4500-J10, K4510 … monash university cio