H9c2 2-1 cells
WebH9c2 cells were incubated with ET-1 in the presence or absence of KMUP-1 for 3 h. In order to measure ROS production induced by ET-1, cells were stained with 10 μM of DCFH-DA for 30 min at 37 °C, and immunofluorescence was visualized using a digital camera attached to an inverted microscope. Photographic images were taken of five random fields. WebAug 3, 2024 · An H9c2 (2-1) cell line, originally derived from embryonic left . ventricular rat heart tissue through the method of selective . serial passaging, (9) was purchased …
H9c2 2-1 cells
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WebJan 6, 2024 · Di bawah ini kami sertakan daftar produk cell line Elabscience. Jika Anda tidak menemukan cell line yang dibutuhkan, mohon untuk menghubungi: Whatsapp 081293185185. Catalogue No. Description. Size. EP-CL-0001. 293 [HEK-293] Cell Line. 1×10? cells/vial. WebOct 7, 2024 · Cells. H9c2 (2-1) rat-cardiac myoblast cells from ECACC were cultured on glass-bottom dishes (D11130H, Matsunami-Glass, Osaka) with DMEM including 1.8 mM CaCl 2 and 0.8 mM MgSO 4 (D6429; Sigma-Aldrich, St. Louis, MO) supplemented with 10% FBS, 100 U/mL penicillin G and 0.1 mg/mL streptomycin, in the 37 °C, 5% CO 2 …
WebPurchase high quality cryopreserved rat BDIX heart myoblast H9c2 cells from ECACC for myoblast differentiation, transfection, cardiomyocyte and muscle biology. US EN. …
WebJul 16, 2024 · Oxygen–glucose deprivation reduced the viability of H9C2 cells. H9C2 cells were deprived of oxygen and glucose for 6 h (incubated in glucose-free medium without fetal bovine serum, in a 1% O 2, 5% CO 2, 94% N 2, and 37 ℃ environments) and reoxygenated for 4 h (incubated in a high-glucose medium containing fetal bovine serum, in a 5% CO 2 ... WebCells were grown at 1.2 × 10 5 in 6-well plates. H9C2 cells were cultured in high glucose (HG, 33 mM) or normal glucose (NG, 5 mM) during 21 days, changing the media each …
WebCell viability was determined by MTT assay, and caspase-3, cleaved caspase-3, Bcl-2, Bax, heat shock protein (Hsp) 70 and Akt levels were assessed by western blotting. A lactate …
WebSep 22, 2015 · For cell isolation, 1-2-day-old rat or mice were sacrificed, hearts were excised, atria were removed and ventricles were minced. ... H9C2 (2–1) (ATCC) rat cardiac muscle cells were cultured in DMEM … documate 3125 one touch installWebIf use of this culture results in a scientific publication, it should be cited in the publication as: H9c2 (2-1) (ECACC 88092904) Skeletal muscle phenotype, no cardiac properties … extremecraft minecraft server ipWebWe conclude that in H9c2 myoblasts, sustained activation of JNK1/2 signalling pathway during oxidative stimulation is followed by an apoptotic phenotype, while transient JNK1/2 activation correlates well with cell survival, suggesting a dual role of this signalling pathway in cell fate determination. Publication types extremecraft server countWebH9c2 (2-1) CRL-1446 ™. H9c2 (2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue that exhibits many of the properties of skeletal muscle. This cell line can be used in cardiovascular disease research. Product category. extremecraft ticketWebFeb 21, 2024 · Briefly, H9c2 cells were incubated with ZYZ-772 at different concentrations for 2 h before possessing to the 1 mM CoCl 2. After 12 h the medium was removed from the wells, added 100 uL MTT (0.5 mg/mL) to each well, and the samples were then incubated for 4 h at 37 °C. extreme creations burleson txWebTransient and sustained oxidative stress differentially activate the JNK1/2 pathway and apoptotic phenotype in H9c2 cells Mol Cell Biochem. 2008 Feb;309(1-2):177-89. doi: … extreme crafting tableWebH9C2 cells would continue to take in Ca2+, decreasing extra-mitochondrial Ca2+levels. Figure 2: The images on the left are the un-differentiated and differentiated H9C2 cells. After differentiation, the cardiac myoblasts turned into “cardiac myocyte-like” cells. Due to mutations that could have occurred, it is hard to tell their exact documation location