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Sfp phosphopantetheinyl transferase

WebWith recombinant Sfp and 16-17-kDa peptidyl carder protein (PCP) domains excised from the SrfB1 and SrfB2 modules as apo substrates, k(cat) values of 56-104 min-1 and K(m) … Web25 May 2012 · The key enzymes needed for their production belong to the family of polyketide synthases (PKSs) and non-ribosomal peptide synthases (NRPSs) that are …

Cyanobacterial Sfp-type phosphopantetheinyl transferases functionalize

WebSmall molecules of diverse structures were conjugated to coenzyme A (CoA) and then covalently attached to the phage displayed PCP by Sfp phosphopantetheinyl transferase. … WebBacillus subtilis surfactin phosphopantetheinyl transferase Sfp produced in E. coli mediated the conjugation of firefly luciferase N-terminally extended by eleven amino acids forming … looking for alaska quotes analysis https://superiortshirt.com

A Single Sfp-Type Phosphopantetheinyl Transferase Plays a Major …

WebSfp-type 4'-phosphopantetheinyl transferase is required for lysine synthesis, tolerance to oxidative stress and virulence in the plant pathogenic fungus Cochliobolus sativus … Web16 Jun 2009 · Function Activates the seven peptidyl carrier protein (PCP) domains of the first three subunits (SrfAA, SrfAB and SrfAC) of surfactin synthetase by transferring the 4' … Web13 Jul 2009 · The cyanobacterium Synechocystis sp. PCC6803 harbours one phosphopantetheinyl transferase (PPTase), Sppt. Protein modelling supported previous … looking for alaska pdf download

Cyanobacterial Sfp-type phosphopantetheinyl transferases functionalize

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Sfp phosphopantetheinyl transferase

Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl ...

Web1 Jan 2014 · Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. … WebSurfactin phosphopantetheinyl transferase (Sfp) represents the second family of PPTases. Sfp is the PPTase necessary for installing PPant on the PCP of surfactin synthase.

Sfp phosphopantetheinyl transferase

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WebBelongs to the P-Pant transferase superfamily. Gsp/Sfp/HetI/AcpT family. Curated. Phylogenomic databases. HOGENOM. CLU_119926_0_0_6; InParanoid. P37623; OMA. … Web19 Oct 2005 · We recently reported the use of peptide carrier proteins (PCPs) for site-specific protein labeling with small molecules by Sfp phosphopantetheinyl transferase ( …

WebHere we report on the inactivation of an A26 Sfp-type 4'-phosphopantetheinyl transferase (Sfp-type PPTase). The inactivation of the gene resulted in loss of NRPs/PKs production. … WebThis enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. It is also known as 4'-phosphopantetheinyl …

WebNational Center for Biotechnology Information Web17 Jun 1999 · The Bacillus subtilis Sfp protein activates the peptidyl carrier protein (PCP) domains of surfactin synthetase by transferring the 4'-phosphopantetheinyl moiety of …

Web27 Jun 2006 · Sfp phosphopantetheinyl transferase covalently attaches small-molecule probes including biotin and various organic fluorophores to a specific serine residue in the …

WebMagnesium ion binding Specific Function Activates the seven peptidyl carrier protein (PCP) domains of surfactin synthase SRF1/2/3 by transferring the 4'-phosphopantetheinyl … looking for alaska pdf free onlinelooking for alaska book coverWeb10 Sep 2024 · Thus, the crystal structure confirms that PcpS is a monomeric Sfp-like PPTase (Sfp: ... Leblanc, C. et al. 4’-Phosphopantetheinyl transferase PptT, ... looking for alaska sceneWeb17 Sep 2013 · We determined the crystal structure of the group II phosphopantetheine transferase Sfp from Bacillus in complex with a substrate carrier protein in the presence … hopscotch coding website videoWeb31 Jan 2014 · The phosphopantetheinyl transferases (PPTases) are responsible for the activation of the carrier protein domains of the polyketide synthases (PKS), non ribosomal … looking for alaska series free downloadWeb22 Jan 1998 · In contrast to other phosphopantetheinyl transferases (PPTases) previously examined, Sfp will modify the apo forms of heterologous recombinant proteins, including … looking for alaska summary chapters tumblrWebIn this review, all current knowledge on this class of enzymes that post-translationally install a 4'-phosphopantetheine arm on various carrier proteins are discussed. Covering: up to … looking for alaska summary chapter 4